Intergene Biosciences Pvt Ltd provides LC-MS (Liquid Chromatography-Mass Spectrometry) which is a powerful analytical technique used for the detection, identification, and quantification of metabolites in various biological samples. It combines the separation capabilities of liquid chromatography with the sensitive and selective detection of mass spectrometry, allowing for comprehensive metabolomic analysis. LC-MS for metabolites and its applications: Principle: LC-MS involves the separation of metabolites through liquid chromatography, followed by their ionization and detection by mass spectrometry. Metabolite samples are first introduced into a liquid chromatography system, where they undergo separation based on their physicochemical properties. The separated metabolites are then directed into the mass spectrometer, where they are ionized and fragmented. The resulting mass spectra are analyzed to identify and quantify specific metabolites. Metabolite Profiling: LC-MS enables the profiling of metabolites present in biological samples, providing a comprehensive snapshot of the metabolome. It allows for the simultaneous detection of a wide range of metabolites, including small molecules such as amino acids, lipids, organic acids, sugars, and secondary metabolites. Metabolite profiling aids in understanding metabolic pathways, identifying biomarkers, and unraveling metabolic changes associated with diseases, drug responses, or environmental factors. Metabolite Identification: LC-MS facilitates the identification of unknown or novel metabolites. By comparing the obtained mass spectra and retention times with reference databases or authentic standards, metabolites can be confidently identified. Additionally, fragmentation patterns obtained during MS/MS analysis provide structural information, aiding in the elucidation of metabolite structures. Quantitative Analysis: LC-MS allows for the accurate quantification of metabolites in biological samples. Isotope-labeled internal standards are commonly used to improve quantitation accuracy. This quantitative approach enables the measurement of metabolite concentrations, metabolic fluxes, and alterations in metabolite levels under different conditions or disease states. Metabolic Profiling in Disease Research: LC-MS-based metabolic profiling plays a crucial role in understanding disease mechanisms, identifying biomarkers, and developing new therapeutic strategies. It aids in characterizing metabolic alterations associated with various diseases, including cancer, cardiovascular diseases, metabolic disorders, neurodegenerative diseases, and infectious diseases. Metabolic profiling can also provide insights into drug metabolism and toxicity. Environmental and Food Analysis: LC-MS is used for the analysis of metabolites in environmental samples, such as water, soil, and air, to monitor pollutants, assess environmental impacts, and study ecological interactions. It is also employed in food science and safety to detect contaminants, evaluate nutritional composition, and assess food quality and authenticity. Drug Metabolism and Pharmacokinetics: LC-MS is extensively used in pharmaceutical research to study drug metabolism and pharmacokinetics. It aids in identifying drug metabolites, determining their concentrations, and understanding metabolic pathways. LC-MS also helps in evaluating drug-drug interactions, assessing bioavailability, and optimizing drug dosage regimens. Method Development and Optimization: LC-MS offers flexibility in method development, allowing for the optimization of chromatographic separations, ionization techniques, and mass spectrometer parameters. This adaptability enables researchers to tailor methods to specific metabolite classes, sample matrices, or research objectives. LCMS#MassSpectrometry#Chromatography#AnalyticalChemistry#Metabolomics#Proteomics#PharmaAnalysis #EnvironmentalAnalysis#OmicsResearch To know more: Contact- 08048040957 / 9902608505 email- codons@intergenebio.com website-www.intergenebio.com